In Vitro Clonal Propagation of Primula Veris L . and Preliminary Phytochemical Analysis
نویسنده
چکیده
A method of in vitro clonal propagation of cowslip Primula veris L. (Primulaceae) is reported. This is a species of medicinal importance, protected by law in Poland. MS medium Murashige and Skoog (1962) with 6 benzyladenine BA (4.44 μM) and 2,4-dichlorophenoxyacetic acid 2,4-D (1.13 μM) was found to be optimal for in vitro cowslip propagation from shoot tips under the conditions of culture. Rooting was best in the presence of indole-3-butyric acid IBA (2.45 μM). In vitro seedlings were transferred to pots and then acclimated. After transplanting to a garden they showed further growth and development, including flowering and fruiting. Phytochemical analysis (2D-TLC) revealed that the flavonoid compounds in leafy shoots from in vitro culture were similar to those in leaves from field cultivation.
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